Sanyo - Mac 1200 Autoclave Manual 'link'

: Avoid overloading the chamber. Leave sufficient space between items for steam to circulate freely, which is critical for complete sterilization. Maintenance & Troubleshooting

According to general autoclave operating procedures and specific MAC 1200 guides: 121˚C 135˚C sanyo mac 1200 autoclave manual

For further information on the Sanyo Mac 1200 autoclave manual, you can: : Avoid overloading the chamber